inos antibodies  (Proteintech)

Journal: Bioactive Materials

Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis

doi: 10.1016/j.bioactmat.2025.10.023

Figure Lengend Snippet: MXMoS 2 DNAgel alleviates inflammation and promotes osteogenesis by modulating macrophage polarization. (A) qRT-PCR analysis of NOS 2 , Il6 , Arg 1 and Il10 levels of RAW264.7 cells after 24h of inflammatory stimulation. (B) ELISA quantification of IL-6 and IL-10 levels of RAW264.7 cells after 24h of inflammatory stimulation. (C – D) Quantitative analysis and representative immunofluorescence staining of iNOS and ARG1. (E) Representative dot plots showing the distribution of iNOS and ARG1 expression in macrophages isolated from POM C57BL/6 mice after DNA hydrogel, MXene/MoS 2 DNA hydrogel, and MXene/MoS 2 DNA hydrogel with NIR irradiation treatment. (F) The stacked bar graphs summarize the proportions of four macrophage subsets (iNOS + ARG1 - , iNOS − ARG1 + , iNOS − ARG1 - , iNOS + ARG1 + ) across groups (n = 3). (G) qRT-PCR analysis of Alp , Runx2 and Sp7 levels of MC3T3-E1 cells after 7 days of osteogenic induction under different treatments. (H) ALP staining of MC3T3-E1 after 7 days. (I) Quantitative analysis of ALP staining. (NC: control group, PC: LPS-treated group) ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

Article Snippet: RAW264.7 cells cultured under different stimulation conditions were fixed with 4 % paraformaldehyde (PFA) for 10 min, followed by permeabilization with 0.25 % Triton X-100 in PBS for 10 min. After washing 3 times with PBS (5 min each), cells were blocked with 1 % BSA for 30 min. After washing, cells were incubated with diluted Mouse Arg1 and iNOS antibodies (Proteintech) at room temperature for 1 h. Following additional PBS washes, cells were incubated with secondary antibodies at room temperature for 1 h in the dark.

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Expressing, Isolation, Irradiation, Control

Journal: Bioactive Materials

Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis

doi: 10.1016/j.bioactmat.2025.10.023

Figure Lengend Snippet: MXMoS 2 DNAgel alleviates inflammation and promotes osteogenesis by modulating macrophage polarization. (A) qRT-PCR analysis of NOS 2 , Il6 , Arg 1 and Il10 levels of RAW264.7 cells after 24h of inflammatory stimulation. (B) ELISA quantification of IL-6 and IL-10 levels of RAW264.7 cells after 24h of inflammatory stimulation. (C – D) Quantitative analysis and representative immunofluorescence staining of iNOS and ARG1. (E) Representative dot plots showing the distribution of iNOS and ARG1 expression in macrophages isolated from POM C57BL/6 mice after DNA hydrogel, MXene/MoS 2 DNA hydrogel, and MXene/MoS 2 DNA hydrogel with NIR irradiation treatment. (F) The stacked bar graphs summarize the proportions of four macrophage subsets (iNOS + ARG1 - , iNOS − ARG1 + , iNOS − ARG1 - , iNOS + ARG1 + ) across groups (n = 3). (G) qRT-PCR analysis of Alp , Runx2 and Sp7 levels of MC3T3-E1 cells after 7 days of osteogenic induction under different treatments. (H) ALP staining of MC3T3-E1 after 7 days. (I) Quantitative analysis of ALP staining. (NC: control group, PC: LPS-treated group) ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

Article Snippet: Primary antibodies for immunocytochemistry and immunofluorescence staining—including ARG1 and iNOS, TRAP, β-Catenin, OCN and WNT4—were obtained from Proteintech (China).

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Expressing, Isolation, Irradiation, Control